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Approximately 95% of primary bile acids are reabsorbed through the terminal ileum sodium-dependent bile acid transporter and enter the enterohepatic circulation, while the remaining 5% are metabolized by bile salt hydrolases and 7α-dehydroxylases secreted by Bacteroides , Clostridium , Bifidobacterium , and Lactobacillus into secondary bile acids such as deoxycholic acid and lithocholic acid (Begley et al., 2005

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To investigate the potential metabolic mechanism(s) behind GPS-mediated therapeutic effects against NAFLD, LC-MS-based serum metabolomics was performed